Glucosinolate Biosynthesis (Further Characterization of the Aldoxime-Forming Microsomal Monooxygenases in Oilseed Rape Leaves)

Subclade of flavin-monooxygenases involved in aliphatic glucosinolate biosynthesis.

Glucosinolates (GSLs) are amino acid-derived secondary metabolites with diverse biological activities dependent on chemical modifications of the side chain. We previously identified the flavin-monooxygenase FMO(GS-OX1) as an enzyme in the biosynthesis of aliphatic GSLs in Arabidopsis (Arabidopsis thaliana) that catalyzes the S-oxygenation of methylthioalkyl to methylsulfinylalkyl GSLs. Here, we...

Reducing Damage to Oilseed Rape by Woodpigeons: I. Trials with Cinnamamide; Ii. Breeding High Glucosinolate Varieties

Partial purification and properties of a microsomal lysophosphatidic acid acyltransferase from oilseed rape

Storage oils in seeds are synthesized via the Kennedy pathway localized in the endoplasmic reticulum (Gurr, 1980). Triacylglycerol is formed by the sequential addition of three acyl groups to a glycerol 3-phosphate backbone. The esterifications are catalysed by three separate acyltransferase enzymes. It is believed that the enzyme catalysing esterification of acyl groups to the sn-2 position of...

Combination of near Infrared Spectroscopy and Multivariate Analysis to Detect Valine (c5h11no2 ) in Oilseed Rape Leaves

Valine (C5H11NO2) is one of the most important amino acids in oilseed rape and has a close relationship with the influence of herbicide stress during growing stage. The traditional methods for amino acid detection were to use high performance liquid chromatography (HPLC) or amino acid analyzer. These methods were time consuming for preparation and detection, costly, laborious and not convenient...

Photorespiratory NH(4)(+) production in leaves of wild-type and glutamine synthetase 2 antisense oilseed rape.

Exposure of oilseed rape (Brassica napus) plants to increasing leaf temperatures between 15 degrees C and 25 degrees C increased photorespiratory NH(4)(+) production from 0.7 to 3.5 micromol m(-2) s(-1). Despite the 5-fold increase in the rate of NH(4)(+) production, the NH(4)(+) concentration in root and leaf tissue water and xylem sap dropped significantly, whereas that in the leaf apoplastic...